Glass Micro Pipette Micro
Injector Holder Perfusion Apparatus
BLM Technique - Brochure
Ultrasonic Cleaner Hamilton
Syringes Precision Resistors
BLM "painting" tools
Glass Micro pipette. This
5 cm long micro pipette is the optimal tool for "painting" of Mueller type
BLMs. It has smooth fire polished tip and can be cleaned with any
solvent or sterilized in flame. PART # GMP-5
Teflon Brush While glass
micropipette is a perfect tool sufficient in most cases for Mueller-Rudin
membrane formation, removal of the excess of lipids from a hole may not
be easy in some cases. For horizontal BLM, it can be done much easier
using miniature Teflon "brush". While it is not exactly "brush" but
a flat Teflon sheet, it is in many aspects better than regular painters
brush used for BLM "painting". Most important, our brushes are made
from chemically inert material so they can be easily washed with any solvent
or sterilized at high temperature. Brush consist of ~0.03 mm thick
diamond shaped Teflon, hot-melt attached to a Teflon tube which is placed
over a stainless steel syringe needle serving as a handle. Each brush
supplied with individual storage container. Part
Has standard 4 mm diameter 100 mm long stainless steel
rod for mounting in almost any micro manipulator. Can hold one or two glass
capillaries 0.6 - 2 mm in diameter.
PART NUMBER: MH-1
This simple inexpensive device is designed for manual
solution exchange in the BLM cell. Since plungers of two syringes
are connected with polymer coated wire, the volume injected into the cell
is equal to the volume taken out. Designed to work with 10-12 cc syringes
but can be easily modified for use with other syringes from 1 to 15 cc.
Dimensions: 210 x 70 mm ~= 8" x 3" (base plate).
PART NUMBER: PA-10A
START ON BLM TECHNIQUE.
This compact but informative brochure
contain all the essential information needed to set up planar bilayer
workstation and begin experimentation in shortest possible time.
A "must have" for the beginners but even experienced researchers may find
some useful information.
Table of Contents
2. Equipment required
3. General recommendations on
setting up BLM workstation.
4. Making Mueller-Rudin BLM
5. Making Montal-Mueller BLM
6. Making horizontal BLM
7. Examples of BLM experiments
membrane activity of proteins or peptides
dependencies of protein incorporation
Dimensional drawing of BLM chamber
BLM chamber cleaning and reassembling.
Circuit Diagram of a basic BLM amplifier
21 page, 12 figures. Currently US $25.00 including USPS shipping within USA
PART NUMBER: QSB-1
Also available in e-book format
This Ultrasonic Cleaner is compact, reliable and can do cleaning
job as well as more expensive devices. We recommend them for cleaning
heavily contaminated BLM chambers, teflon partitions, and other small parts
that heed cleaning on hard to access surfaces. While we did not test cleaners
ability to make 30nm liposomes, they are suitable for basic lipid re-suspension,
which is a part of several vesicle preparation techniques. UC-1A
require 120V AC , UC-1E is for 220 V AC.
Tank Size, ml
These syringes are perfect for adding small volume of
solution to BLM chambers. Since they are much small, it is often
more convenient to use them instead of than any automatic pipettes
in a tight space around BLM chamber. Commonly used sizes of 10 ul
and 50 ul are available
PART NUMBERs: HS-10; HS-50
These parts are useful for testing or calibration of electrophysiological
amplifiers. Commonly used 1000 & 100 mOhm, 1% are available
PART NUMBERs: GR-1000; GR-100
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