Video-BLM Systems -- Video-Setup
for Horizontal BLM -- Video
fluorescence BLM System.
Researchers interested to work on planar phospholipid
bilayers (BLM) facing a difficult task of assembling an experimental setup
from many pieces of electronic, mechanical and optical equipment.
Eastern Scientific is offering a line of products that can be integrated
into complete system for BLM experiments. Your can get everything
you need for less than $2000* For those
who have little or no experience with BLM we suggest relatively simple
Basic BLM System shown in the photograph below.
* Basic configuration: BVS-130C
Some assembly required. Recording device (Computer interface, stirrer,
etc) not included. If magnetic stirrer is required we recommend
model with RH-12M or RH-25
RSSM. If standard air table is not available, VIS-xx
antivibration stands are recommended.
for Vertical BLM
Our basic setup for video microscopy of BLMs includes the
1. Metal Enclosure (FC-38A or
Cage), H-28 x W-28 x L-38 cm (11" x 11" x 15") is assembled from aluminum
(A) or Composite (C - aluminum foil over expanded plastic) panels and mounted
on a solid aluminum base plate. Two doors can be widely open closed for maximum screening of electromagnetic interference.
This enclosure can be used for a variety of electrophysiological application
other than BLM. If you plan to add a lot of your own components to
the system, we recommend upgrade with optional Bench
Plate that has a number of treaded holes on a square pattern (Metric
or English). These bases ensure great flexibility for installing
2. Mounting hardware for amplifier headstage.
3. Mounting hardware for two syringes. Syringes are used for injecting solution
in BLM cell as required
for making "solvent free" membranes.
4. Video microscope which include video camera* and 4X, 0.1 NA achromatic lens
with at least 12 mm working distance. Overall magnification depends on the size of a video
monitor and is about 250X for 12" monitor screen. Higher
magnification/resolution can be easily achieved with more powerful lenses.
Camera system is mounted on a linear positioner with 15+ mm travel along
5. Illumination system consisting of a LED based light
source and mounting hardware.
6. BLM cell positioner. Equipped with cell holder for
either BC-20 or BC-30
cells. Has 15+ mm travel along Y axis, and 10+ mm along Z axis.
7. Power supply for video camera and light source.
PART NUMBER: BVS-138XY
(XY stands for:
X- enclosure material, Y - type of video camera. When ordering, please
substitute X with A for aluminum or C for composite; Y with B
for B/W analog video or U for USB color computer camera*. Those are
standard options. If you need something special like higher resolution
camera or enclosure with extra thermal insulation please contact us for a
Image above left show an example of BLM rig (with
enclosure removed) containing the following components: 1. 30x40 cm base plate
with M4 holes on 30 mm square pattern; 2. XZ positioner* with BC-20A chamber in
the chamber holder; 3. Y- videoscope positioner with USB camera and 4X
lens; 4. Mounting hardware with HS-1 headstage and EAG-01 electrodes; 5.
Mounting hardware with two 3 cc syringes; 6. LED light source with
battery-powered intensity regulator (normally mounted on a side
wall). A compact version
of this rig is also available (shown above right, composite enclosure).
Includes same component as standard one but placed inside 6" high enclosure
mounted on 8"x12" base (15x20x30 cm). Despite small size this system
is almost as convenient as larger one. If space is
is a solution.
* - Coarse positioners shown here are standard and sufficient for use with low
magnification lenses. Higher magnification will benefit from optical grade
translation stages but price will increase significantly.
for Horizontal BLM
For those who work with horizontal BLMs we have designed
video microscopy system that is significantly more convenient than rigs
build around a regular inverted microscope. It includes the following components:
1. Faraday Cage (FC-30C
with BP-2030 base plate).
2. Inverted video microscope which include video camera* and 4X, 0.1 NA achromatic lens.
magnification depends on the size of a video monitor and
is about 250X for 12" monitor screen. Higher magnification/resolution can
be achieved with more powerful objective lenses.
3. Illumination system consisting of a LED light source
and mounting hardware.
4. Mounting hardware for amplifier headstage.
5. Holder for BCH-1A cell with
mounting hardware that allow +/- 3 mm X-Y and +/-5 mm Z axis (height)
6. Two electrode holders.
7. Power supply for video camera and light source.
PART NUMBER: BVH-130XY
fluorescence BLM System.
FVS-160 optical system shown
here (with enclosure removed) is designed for most advanced studies on
planar lipid bilayers (BLM). Left side of the photograph above is
similar to introduced earlier BVS typr video BLM system, and used for membrane
observation in transmitted light. Right side is a custom made fluorescent
microscope. This system allows combined electrical and high resolution
optical measurements of membrane parameters. Like other BLM setups,
it is assembled on a solid aluminum base plate providing great flexibility
and easiness in installing additional components. Setup includes:
1. Beamsplitter system with precision stage mechanism
for high power objective lens (Fig.1).
2. Arc lamp housing and mounting hardware.
3. Linear low interference arc lamp power supply.
4. Aluminum enclosure (Faraday cage).
5. 12"x24" (or 12"x30") base plate with treaded
(1/4-20 tpi) holes.
6. Four rubber foam or sorbotane anti vibration legs.
7. Video-camera mounting hardware.
Other components are almost identical to used in BVS-1XX
rig and include:
4. Power supply for CCD video camera.
1. Mounting hardware for amplifier headstage.
2. Mounting hardware for two syringes.
3. Video microscope (Fig. 2) which include video camera* and 4X, 0.1 NA, 14 mm
w.d. achromatic lens. Overall magnification depends on the size of
a video monitor (not included) and is about 250X for 12" monitor screen.
Higher magnification/resolution can be easily achieved with more powerful
lenses. Camera system is mounted on a linear positioner with 15+
mm travel along X axis.**
5. BLM cell positioner (Fig.3). Equipped with cell holder
for 20 mm wide BLM cells. Allows cell to be
moved 15+ mm along Y axis, and 10+ mm along Z axis.
We also offering a simplified and less expensive version
of this setup, FVS-138XY, FVS-138XY .
In this modification, video microscope shown in Fig. 2, is replaced with
LED based light source. It allows to use one optical system for both
bright field membrane observation, and for fluorescent microscopy.
In most cases the only inconvenience of this configuration is the absence
of low magnification of BLM imaging during BLM formation.
Some components required for these setups need to be purchased
1. 25 mm diameter excitation and emission filters.
2. 20x28 dichroic beamsplittter.
3. High power objective lens.
4. HBO-100 arc lamp*.
5. Intensified CCD camera.
* If you will be using 100W mercury arc lamp. Other lamps
may require housing modifications and power supply from other source.
PART NUMBER: FVS-160XY or FVS-138XY
* - Two standard camera options are available (Same price):
1. 1/3" CCD size format (510x492 pixel sensor),
monochrome (RS-170 video format) TV camera with 0.1 Lux sensitivty. 2. 1.3 MP
(1280 x 960 pixel sensor) or better Windows compatible color
USB camera. With option 1 you will need a video monitor that can be
either professional B/W monitor or any TV set that have a video input.
Option 2 require a computer with USB port available. In terms of
image quality, the difference is minimal. However, if you plan to do
something more than just observing BLM formation, please make a note that B/W
camera is faster and more light sensitive than color one.
Back to the
top of the page
Due to small volume production and continuous improvements, images here
may be a bit different from currently available products.
BLMrigs.html Date of
latest modification: 10-09-2019